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KMID : 1145720170050010033
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Korean Herbal Medicine Informatics
2017 Volume.5 No. 1 p.33 ~ p.39
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A Case Report of New Commercial Adulterant of Araliae Continentalis Radix Based on the Analysis of ITS2 DNA Barcode Sequences
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Moon Byeong-Cheol
Kim Wook-Jin
Choi Go-Ya
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Abstract
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Araliae Continentalis Radix has been known as a representative herbal medicine distributed with inauthentic adulterants such as the roots of Angelica biserrata and Levisticum officinale. In this study, we identified a new additional commercial adulterant used as Araliae Continentalis Radix. To define the taxonomic origin of the new commercial adulterant, we analyzed ITS2 sequence similarity and identity using NCBI BLAST. The BLAST analysis revealed that the new commercial adulterant belong to the genus Gypsophila but not identical to any plant species. To verify the accurate species, we compared ITS2 sequences of the new commercial adulterant and related taxa including Ar. continentalis, An. biserrata, L. officinale, G. oldhaniana, G. pacifica, and other Gypsophila species and confirmed the new commercial adulterant was the root of G. facifica. These results provide an useful information to prevent the adulteration of Araliae Continentalis Radix and suggest an effective method to verify the origin of commercial herbal medicines.
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KEYWORD
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Araliae Continentalis Radix, Commercial Adulterant, ITS2, Molecular Authentication, Gypsophila facifica
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